ABSTRACT
Leishmania RNA Virus (LRV, Totiviridae) infect Leishmania cells and subvert mice immune response, probably promoting parasite persistence, suggesting significant roles for LRV in host-parasite interaction. Here we describe a new LRV1-4 purification protocol, enabling capsid visualization by negatively stained electron microscopy representing a significant contribution to future LRV investigations.
Subject(s)
Leishmaniavirus/isolation & purification , Virion/isolation & purification , Virology/methods , Leishmaniavirus/ultrastructure , Microscopy, Electron, Transmission , Staining and Labeling/methods , Virion/ultrastructureABSTRACT
Leishmania RNA virus (LRV) has been shown to be a symbiotic component of Leishmania parasites in South America. Nested retro-transcription polymerase chain reaction was employed to investigate LRV1 presence in leishmaniasis lesions from Brazil. In endemic areas of Rio de Janeiro (RJ), no LRV1 infection was observed even with mucosal involvement. LRV1 was only detected in Leishmania (V.) guyanensis cutaneous lesions from the northern region, which were obtained from patients presenting with disease reactivation after clinical cure of their primary lesions. Our results indicated that the severity of leishmaniasis in some areas of RJ, where Leishmania (V.) brazi-liensis is the primary etiological agent, was not associated with Leishmania LRV1 infection.
Subject(s)
Female , Humans , Leishmania braziliensis/virology , Leishmaniasis, Cutaneous/parasitology , RNA Viruses/genetics , Brazil , Polymerase Chain Reaction , RNA Viruses/classification , RNA, Viral/genetics , Severity of Illness IndexABSTRACT
Objective To discuss the 3D reconstruction dual-source CT angiography (DSCT) in diagnosing nutcracker syn-drome,Methods 24 cases with known or suspected "nutcracker" syndrome were underwent with DSCTA and 3D recon-struction, left renal vein (LRV) were demonstrated and measured. Results Among 24 cases, 16 cases were diagnosed with "nutcracker" syndrome; the imaging demonstrated LRV were compressed. The angle between SMA and AO of 24 cases with "nutcracker" syndrome was abnormally acute(<45°). The angle A was 14°～38°, the average angle was 28.12°±7.92°. The angle A' was 11°～21°, the average angle was 16.46°±3.42°. Conclusion The 3D reconstruction DSCTA may be the most useful tool in diagnosing nutcracker phenomenon.
ABSTRACT
Objective To discuss the 3D reconstruction dual-source CT angiography (DSCT) in diagnosing nutcracker syn- drome. Methods 24 cases with known or suspected "nutcracker" syndrome were underwent with DSCTA and 3D recon- struction, left renal vein (LRV) were demonstrated and measured. Results Among 24 cases, 16 cases were diagnosed with " nutcracker" syndrome; the imaging demonstrated LRV were compressed. The angle between SMA and AO of 24 cases with "nutcracker" syndrome was abnormally acute(